lee011 powder  (MedChemExpress)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

doi: 10.1016/j.biopha.2019.108602

Figure Lengend Snippet: Fig. 2. LEE011 inhibits cell proliferation and promotes apoptosis by targeting the Rb-E2F1 pathway in C33A but not HeLa. A. C33A and HeLa cells were treated with increasing concentrations of LEE011, and the morphologic changes of cells were observed by microscopy after 48 h of LEE011 treatment (the magnification is 200X, scale bar, 100 μm). B. The expression of proteins in Rb-E2F1 pathway and apoptosis in C33A cells were detected by western blotting after 2 days of LEE011 treatment. C. The expression of proteins in Rb-E2F1 pathway and apoptosis in HeLa cells were detected by western blotting after 2 days of LEE011 treatment.

Article Snippet: :434.54 g/mol, HY-15777,MCE) was solubilized in 2.3013 ml of DMSO and 10 mM stock solution was made,2 mg of LEE011 powder was solubilized into 20 mM stock solution.

Techniques: Microscopy, Expressing, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

doi: 10.1016/j.biopha.2019.108602

Figure Lengend Snippet: Fig. 3. LEE011 induces cell cycle G1 arrest in C33 A but not HeLa. A.B. After treatment of C33A cell lines with LEE011 (10 μM) during 48 h, G0/G1 portion increased with 19.23% (P < 0.05), S portion decreased with 9.68% (P < 0.05), and G2/M portion decreased with10.18% (P < 0.05). C.D. After treatment of HeLa cell lines with LEE011 (10 μM) during 48 h, there are no significant changes in the cell cycle process.

Article Snippet: :434.54 g/mol, HY-15777,MCE) was solubilized in 2.3013 ml of DMSO and 10 mM stock solution was made,2 mg of LEE011 powder was solubilized into 20 mM stock solution.

Techniques:

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

doi: 10.1016/j.biopha.2019.108602

Figure Lengend Snippet: Fig. 4. LEE011 induces cell apoptosis in C33A but not HeLa. A.B. After treatment of C33A cell lines with LEE011 (10 μM) during 48 h, the apoptotic index increased 2.24 times more (31.7%) than control (14.13%) in C33A (P < 0.05). Alive cells are shown in the lower left part of the panel (Q3); Early apoptotic cells are shown in the lower right part of the panel (Q4); Late apoptotic cells are shown in the higher right part of the panel (Q2); Necrotic cells are shown in Q1. Apoptotic cells were exhibited as annexin V + cells. C.D. In HeLa cells, treatment with 10 μM of LEE011 resulted in apoptosis augmentation with no significant changes compared with control. Alive cells are shown in the lower left part of the panel (Q8); Early apoptotic cells are shown in the lower right part of the panel (Q7); Late apoptotic cells are shown in the higher right part of the panel (Q6); Necrotic cells are shown in Q5. Apoptotic cells were exhibited as annexin V + cells.

Article Snippet: :434.54 g/mol, HY-15777,MCE) was solubilized in 2.3013 ml of DMSO and 10 mM stock solution was made,2 mg of LEE011 powder was solubilized into 20 mM stock solution.

Techniques: Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

doi: 10.1016/j.biopha.2019.108602

Figure Lengend Snippet: Fig. 5. The effects of LEE011 treatment on tumor growth in vivo. A. After euthanasia, tumors were extracted from both of control group and LEE011 treated group. The sizes of tumors extracted from LEE011 group were smaller than the control group in C33A xenograft models. However, the sizes of tumors in the control group and LEE011 treated group had no difference in HeLa Xenografts. B–C. Treated with 200 mg/kg, LEE011 significantly inhibited tumor growth in C33A xenograft models (P < 0.05). Tumor growth had no change in HeLa xenograft models.D.LEE011 had no effect on body weight in C33A xenografts, but caused the HeLa models lost weight. E. The antiproliferative effect of LEE011 was confirmed by Ki- 67 staining in C33A xenografts, and suppression of the RB-E2F1 pathway was confirmed by immunohistochemical staining for CDK4, CDK6, cyclin D1 and Rb. LEE011-induced apoptosis was detected by using TUNEL assay (scale bar, 100 μm). F. The index of the antiproliferative effect, the RB-E2F1 pathway and apoptosis in HeLa xenografts did not show obvious differences. G.H.I. The biochemical indicators (ALT, AST, ALB, ALP, BUN, CR, LDH-L, CK) were detected from blood samples and the morphologic of heart, liver and kidney were studied and showed that there were no serious adverse effects in xenograft models treated with LEE011.

Article Snippet: :434.54 g/mol, HY-15777,MCE) was solubilized in 2.3013 ml of DMSO and 10 mM stock solution was made,2 mg of LEE011 powder was solubilized into 20 mM stock solution.

Techniques: In Vivo, Control, Staining, Immunohistochemical staining, TUNEL Assay